Tuesday 16 August 2022 - 02:46
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Reminder: Genuine LC Laboratories® brand PMA, which we have been manufacturing in the U.S. for more
than 40 years, is available directly from us: Cat No. P-1680 Phorbol 12-Myristate 13-Acetate, >99.5%.
Our PMA is also available from our >50 U.S. and international distributors, and from more than a dozen
major biochemical reagent "resellers" (who sell our PMA under their own brand names and labels)
FREE SHIPPING TO THE U.S. AND 32 OTHER COUNTRIES! [Read more].
Y-5301 Y-27632, Dihydrochloride Salt, >99%
Synonyms : [Y27]

- Size
- US $
- €
- £
- ¥
- 5 mg
- 37
- 36
- 30
- 4,900
- In stock
- 10 mg
- 55
- 53
- 45
- 7,300
- In stock
- 25 mg
- 113
- 110
- 93
- 15,000
- In stock
- 50 mg
- 182
- 178
- 150
- 24,100
- In stock
- 100 mg
- 310
- 303
- 256
- 41,100
- In stock
- 200 mg
- 475
- 465
- 392
- 63,000
- In stock
- 250 mg
- 570
- 558
- 471
- 75,600
- In stock
- 500 mg
- 998
- 978
- 825
- 132,400
- In stock
- 1 g
- 1,590
- 1,558
- 1,314
- 211,000
- In stock
Note: Our Euro, Pound, and Yen prices are revised regularly to account for currency exchange rate fluctuations.
- M.W. 320.26
- C14H21N3O•2HCl
- [129830-38-2]
Storage: Store at or below -20 ºC. Solubility: Soluble in DMSO at 160 mg/mL; soluble in ethanol at 12 mg/mL with warming; soluble in water at 90 mg/mL; buffers, serum, or other additives may increase or decrease the aqueous solubility. Disposal: A.
- More than 150 labs worldwide have purchased Y-27632 from LC Labs (either directly from us or from our many distributors, many of whom resell under their own labels).
- Y-27632 is a novel and specific Rho-associated coiled-coil forming protein kinase (ROCK) inhibitor. Narumiya, S., et al. "Use and properties of ROCK-specific inhibitor Y-27632." Methods Enzymol. 325: 273-284 (2000).
- This research compound is the dihydrochloride salt form of Y-27632. We also plan to offer the free base form; please see Y-27632, Free Base, Cat. No. Y-5399.
- Y-27632 inhibited ROCK-I (Ki = 0.22 µM) and ROCK-II (Ki = 0.30 µM) by competing with ATP for its binding to the kinase. Ishizaki, T., et al. "Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases." Mol. Pharmacol. 57: 976-983 (2000).
- Y-27632 selectively inhibited Ca2+ sensitization of smooth muscle and blocked its contraction, prevented Rho-induced, p160ROCK-mediated formation of stress fibers in cultured cells, and dramatically lowered hypertension in several hypertensive rat models. Uehata, M., et al. "Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension." Nature 389: 990-994 (1997).
- Y-27632 inhibited cell transformation mediated by RhoA and its guanine-nucleotide exchange factor (GEF). Sahai, E., et al. "Transformation mediated by RhoA requires activity of ROCK kinases." Curr. Biol. 9: 136-145 (1999).
- Y-27632 inhibited both Rho-mediated activation of actomyosin and invasive activity of rat MM1 hepatoma cells. Continuous delivery of Y-27632 largely prevented the dissemination of MM1 cells implanted into the peritoneal cavity of syngeneic rats. Itoh, K., et al. "An essential part for Rho-associated kinase in the transcellular invasion of tumor cells." Nat. Med. 5: 221-225 (1999).
- Y-27632 blocked chemotactic peptide-induced development of cell polarity and locomotion, and inhibited myosin light chain phosphorylation with similar potency (ED50 = 0.5-1.1 µM). Niggli, V. "Rho-kinase in human neutrophils: a role in signalling for myosin light chain phosphorylation and cell migration." FEBS Lett. 445: 69-72 (1999).
- Y-27632 had antinociceptive properties, possibly by inhibition of Rho-kinase. Buyukafsar, K., et al. "Rho-kinase inhibitor, Y-27632, has an antinociceptive effect in mice." Eur. J. Pharmacol. 541: 49-52 (2006).
- Y-27632 blocked the development of ischemia/reperfusion-induced acute renal failure, possibly by inhibiting myeloperoxidase activity in an early phase after reperfusion. Teraishi, K., et al. "Preventive effect of Y-27632, a selective Rho-kinase inhibitor, on ischemia/reperfusion-induced acute renal failure in rats." Eur. J. Pharmacol. 505: 205-211 (2004).
- Y-27632 prevented intrahepatic metastasis of human hepatocellular carcinoma. Takamura, M., et al. "Inhibition of intrahepatic metastasis of human hepatocellular carcinoma by Rho-associated protein kinase inhibitor Y-27632." Hepatology 33: 577-581 (2001).
- Y-27632 inhibited both of lysophosphatidic acid (LPA)- and fibronectin (FN)-induced migration and morphological change of rat ascites hepatoma (MM1) cells. It impaired LPA- and FN-evoked formation of focal adhesions and actin bundles and suppressed LPA- and FN-induced tyrosine phosphorylation of focal adhesion kinase and paxillin in MM1 cells. Imamura, F., et al. "Y-27632, an inhibitor of rho-associated protein kinase, suppresses tumor cell invasion via regulation of focal adhesion and focal adhesion kinase." Jpn. J. Cancer Res. 91: 811-816 (2000).
- This Y-27632 product is the dihydrochloride salt, whose CAS number is given above. The CAS number of the free base form is 146986-50-7.
- Related CAS number: 331752-47-7 for Y-27632 diHCl salt monohydrate.
- A CAS number previously assigned to Y-27632 diHCl salt monohydrate, namely 203911-23-3, has been deleted by CAS and is no longer in use, and a CAS number previously assigned to Y-27632, Free Base, namely 742043-06-7, has also been deleted by CAS and is also no longer in use.
- As of October 2013, many other vendors (Adipogen, Affix, AG Scientific, BioVision, Calbiochem/EMD, Cellagen, Enzo, MyBioSource, Reagents Direct, Santa Cruz, VDM Biochemicals, etc.) are incorrectly using CAS number 146986-50-7 for the diHCl or diHCl monohydrate salt forms of Y-27632. Chemical Abstracts Service has assigned the CAS number 146986-50-7 to the free base form only.
- Sold for laboratory or manufacturing purposes only; not for human, veterinary, food, or household use.
- This product is offered for R&D use in accordance with (i) 35 USC 271(e)+A13(1) in the U.S.; (ii) Section 69.1 of Japanese Patent Law in Japan; (iii) Section 11, No. 2 of the German Patent Act of 1981 in Germany; (iv) Section 60, Paragraph 5b of the U.K. Patents Act of 1977 in the U.K.; (v) Sections 55.2(1) and 55.2(6) and other common law exemptions of Canadian patent law; (vi) Section 68B of the Patents Act of 1953 in New Zealand together with the amendment of same by the Statutes Amendment Bill of 2002; (vii) such related legislation and/or case law as may be or become applicable in the aforementioned countries; and (viii) such similar laws and rules as may apply in various other countries.
- Not available in some countries; not available to some institutions; not available for some uses.